Dr. Marco Tulio Núñez
Grado:
Bioquímico, Universidad de Chile, 1972.
Tema de investigación o interés:
• Estudio de los procesos de endo y exocitosis del transportador DMT1 en la regulación de la absorción intestinal de hierro.
• Mecanismos moleculares de la hemocromatosis hereditaria.
• Hierro y cobre en la producción de estrés oxidativo y envejecimiento celular.
• Desregulación de la homeostasis del hierro en la enfermedad de Parkinson.
• Homeostasis del hierro en la función neuronal.
Distinciones y premiaciones:
•Young Investigator Research Award, National Institute or Arthritis, Diabetes, Digestive and Kidney Diseases.
•Beca de Período Sabático Fundación Andes.
•Cátedra Presidencial en Ciencia.
•Evaluador Externo, Department of Medicine Okayama University, Okayama, Japón.
•Evaluador NHI-NIGMS Program Project Grant Mechanisms of Metal Ion Regulation in Human Cells.
•Miembro Correspondiente de la Academia Chilena de Ciencias.
Proyectos:
•Instituto Milenio en Dinámica Celular y Biotecnología
Proyecto Iniciativa Científica Milenio Mideplan P001-05-F
Investigador clave
Duración: 2006 – 2015
•Posicionamiento de los transportadores DMT1 y ferroportina en la membrana apical y basolateral y la regulación de la absorción intestinal de hierro.
FONDECYT 1070840. Investigador Responsable
Duración: 2007 - 2010
•Estudios del metabolismo del hierro en la enfermedad de Parkinson”
Proyecto de colaboracion CONICYT/INSERM
Investigadores responsables: Prof. Marco Tulio Nunez y Prof. Etienne Hirsch
Duración: 2007 - 2010
Publicaciones:
•Arredondo M., Cambiazo V, Tapia L, González-Aquero M, Nuñez M.T,
Uauy R., González M.
“Copper overload affects copper and iron metabolism in Hep-G2 cells”
J. Physiol Gastrointest Liver Physiol 287: G27 – G32 (2004)
•Mazariegos, D.I., Pizarro, F., Olivares, M., Nunez, M.T. y Arredondo, M. 2004.
Fe bis-glycine chelate and Fe-ascorbate share similar regulatory absorption mechanisms in Caco-2 cells
J. Nutr. 134: 395-398 (2004)
•Zambrano, C., Egaña, J.T., Núñez, M.T., Maccioni, R.B. and Gonzalez-Billault, Ch.
Decreased activity of cdk5 in hippocampal cells under oxidative stress is responsible for reduced tau phosphorylation
Free Radical Biology & Medicine 36: 1393-1402 (2004)
•Rodríguez, D.A., Moncada, C., Núñez, M.T., Lavanderos, S., Ponnappa, B.C. Israel, Y.
Ethanol increases tumor necrosis factor-alpha receptor-1 (TNF-R1) levels in hepatic, intestinal and cardiac cells
Alcohol 33: 9-15 (2004)
•Núñez, M.T., Gallardo, V., Muñoz, P., Tapia, V., Esparza, A., Salazar, J. & Speisky, H.
Progressive iron accumulation induces a biphasic change in the glutathione content of neuroblastoma cells
Free Rad Biol & Med 37: 953-960 (2004)
•Inestrosa, N.C., Columbres, M., Núñez, M.T. and Morgan, C.
Structure and function of amyloid in Alzheimer’s disease
Progress in Neurobiology 74: 323-349 (2004)
•Aguirre, P., Mena, N., Arredondo, M., Tapia, V. and Núñez, M.T.
Iron homeostasis in neuronal cells: a role for SCL40
BMC Neuroscience 6, article 3 (2005)
•Ezquer, F., Nuñez, M.T. and Israel, Y.
Hemochromatosis: Antisense gene against the iron transporter DMT1 inhibits iron uptake in intestinal cells and shows RNAi and antisense effects
Biochem. Pharmacol. 69: 1559-1566 (2005)
•Arredondo, M., Núñez, M.T.
Iron and copper metabolism. (Review)
Molecular Aspects of Medicine 26: 313-327 (2005)
•Arredondo, M., Tapia, V., Muñoz, P., Aguirre, P., Rojas, A., Marzolo, M.P. and Núñez, M.T.
Apical distribution of 2-microglobulin is associated with inhibition of apical iron uptake inbHFE- intestinal epithelia cells
Biometals 19: 379-388.(2006)
•Aracena, P., Aguirre, P., Muñoz, P. and Núñez, M.T.
Iron and glutathione at the crossroad of redox metabolism in neurons
Biological Research 39: 157-165 (2006).
•Ezquer, F., Núñez, M.T., Rojas, A. Asenjo, J. and Israel, Y.
Hereditary hemochromatosis: an opportunity for gene therapy
Biological Research 39: 113-124 (2006)
•Arredondo, M., Martinez, R., Núñez, M.T., Ruz, M., and Olivares, M. 2006
Increasing concentrations of Fe, Cu or Zn inhibit Fe and Cu uptake
Biological Research 39: 191-193 (2006)
•Mazariegos DI, Cabantchik, IZ, Cassels BK and Núñez MT.
Characterization of mitochondrial iron uptake in HepG2 cells
Biological Research 39: 199-201 (2006)
•Mena NP, Esparza, AL and Núñez MT.
Regulation of transepithelial transport of iron by hepcidin
Biological Research 39: 191-193 (2006)
•Aguirre, P., Mena NP, Tapia V, Rojas A, Arredondo M and Núñez MT.
Homeostatic and antioxidant responses of neuroblastoma cells to high iron supply
Biological Research 39: 103-104 (2006)
•Aracena P, Aguirre P, Muñoz P, Núñez MT.
Iron and glutathione at the crossroad of redox metabolism in neurons.
Biological Research 39:157-165 (2006)
•Muñoz P, Zavala G, Castillo K, Aguirre P, Hidalgo C, and Núñez MT.
Effect of iron on the activation of the MAPK/ERK pathway in PC12 neuroblastoma cells
Biological Research 39: 189-190 (2006)
•Salazar, J., Mena, N. and Núñez, M.T.
Iron dyshomeostasis in an early stage model of Parkinson’s disease
Journal of Neural Transmition 71(Suppl.): 205-213 (2006)
•Mura, C.V., Delgado, R., Aguirre, P., Bacigalupo, J. and Nuñez, M.T.
SHSY5Y neuroblastoma cells survival to iron challenge results in a quiescent and functional cell population
Journal of Neurochemistry 98:11-19 (2006)
•Hidalgo C, Núñez MT.
Calcium, iron and neuronal function.
IUBMB Life 59(4-5): 280-285 (2007)
•Hidalgo, C., Carrasco, A., Muñoz, P. and Núñez, M.T.
A role for reactive oxygen/nitrogen species and iron on neuronal synaptic plasticity
Antioxidants and Redox Signalling 9: 245-255 (2007)
•Aguirre, P., Valdés, P., Aracena-Parks, P. and Núñez, M.T.
Up-regulation of glutathione and gamma-glutamate-cysteine ligase is part of the long-term adaptation to iron accumulation in neuronal SH-SY5Y cells
American Journal of Physiology (Cell Physiol) 292:C2197-C2203 (2007)
•Mena NP, Esparza A, Núñez MT. 2008. Hepcidin inhibits DMT1 expression and regulates transepithelial iron transport in Caco-2 cells and rat duodenum.
American Journal of Physiology (Gastrointestinal and Liver Physiol) 294(1):G192-198 (2008)
•San Martin, C., Theil, E., Garri, C., Pizarro, F., Walter, T. and Núñez, M.T.
Clathrin-mediated apical endocytosis of soybean ferritin by Caco-2 cells.
Journal of Nutrition 138:659-666 (2008)
•Salazar, J., Mena, M., Hunot, S., Prigent, A., Alvarez-Fischer, D., Arredondo, A., Duyckaerts, C., Zhao, L., Garrick, L.M., Nuñez, M.T., Garrick, D.M., Raisman-Vozari, R., and Hirsch, E.C.
Divalent metal transporter 1 (DMT1) contributes to neurodegeneration in parkinsonian syndromes.
Proceedings of the National Academy of Sciences USA 105(47):18578-83 (2008).
•Juan Pablo Muñoz, Hernán Alcaino, Jessica Díaz-Elizondo, Claudio Olea, Mario Chiong, Zully Pedrozo, Marco Tulio Núñez, Cecilia Hidalgo and Sergio Lavandero
Inducible Nitric Oxide Synthase and Redox Signaling Mediate Cardiomyocyte Survival and Hypertrophy Stimulated by Iron.
Submitted, Journal of Biological Chemistry (2008).
•Barbeito, A. G., Garringer, H. J., Baraibar, M.A., Gao, X., Arredondo, M., Núñéz, M.T., Smith, M.A, Ghetti, B., Vidal, R.
Abnormal iron metabolism and oxidative stress in mice expressing a mutant form of the ferritin light polypeptide gene.
Journal of Neurochemistry 109(4):1067-1078 (2009).
•Pablo Muñoz-Carvajal, Alexis Humeres, Alfredo Kirkwood, Cecilia Hidalgo and Marco T. Núñez.
An iron-calcium connection in NMDA receptor signaling and hippocampal synaptic plasticity
En revisión, Journal of Biological Chemistry (2009).
•Marco T. Núñez, Victoria Tapia, Alejandro Rojas, Pabla Aguirre, Francisco Gómez, and Francisco Nualart.
Iron supply determines apical/basolateral membrane distribution of intestinal iron transporters DMT1 and ferroportin
American Journal of Physiology - Cell Physiology en prensa 294: C000-C000, (2010)
•Haeger, P., Alvarez, A., Núñez, M.T. and Hidalgo, C.
Selective stimulation of hippocampal DMT1 1B and +IRE mRNA splicing variants and enhanced DMT1 protein expression after NMDA receptor stimulation or spatial memory training.
Neurotoxicity Res. Aug 5 (2009) [Epub ahead of print].
•Juan Pablo Muñoz, Hernán Alcaino, Jessica Díaz-Elizondo, Claudio Olea, Mario Chiong, Zully Pedrozo, Marco Tulio Núñez, Cecilia Hidalgo and Sergio Lavandero.
Inducible Nitric Oxide Synthase and Redox Signaling Mediate Cardiomyocytes Survival and Hypertrophy Stimulated by iron.
Free Radical Biology & Medicine Dec 4 (2009) [Epub ahead of print].