Grado:

Ph.D., University of Wisconsin, USA, 1968

Tema de investigación o interés:

• Rutas y mecanismos involucrados en el traslado y establecimiento de dominios citoplasmáticos en el zigoto y embrión temprano de la sanguijuela y del pez

Cebra.

Para este propósito se utilizan diferentes tipos de video microscopía digital, confocal y electrónica  en combinación con inmunofluorescencia, hibridación in situ

y microinyección de sondas, drogas y anticuerpos.

• Reorganización del citoesqueleto de glándulas salivales labiales de pacientes con el síndrome de Sjögren (enfermedad auto inmune). Inmonofluorescencia y

bioquímica.

• Desarrollo de nuevas metodologías para la preparación de células y tejidos destinados a la inmunofluorescencia o hibridación in situ.

Research Interest:

• Pathways and mechanisms involved in the transport and establishment of cytoplasmic domains in  the leech and zebrafish zygote and early embryo.

To this purpose different types of digital video microscopy, confocal and electron microscopy are utilized in combination with immunofluorescence, in situ

hybridization and microinjection of probes, drugs and antibodies.

• Reorganization of the cytoskeleton in the labial salivary glands of patients with the Sjögren syndrome (autoimmune disease).  Immunofluorescence and

biochemistry.

• Development of new methodologies for the preparation of cells and tissues destined for immunofluorescence or in situ hybridization.

Distinciones y premiaciones:

• Rockefeller Foundation Fellow.

• Guggenheim Fellow.

• Research Biologist. University of California, Berkeley. California. USA.

• Vising Professor. Case Western Reserve University. Cleveland, Ohio. USA.

• Visiting Professor. Biocenter. University of Basel. Basel. Switzerland.

• Visiting Professor. Sissa. Trieste, Italy.

Proyectos:

• Tecnofarma, Novartis Chile, Grunental Chilena Ltda.

Publicaciones:

•Fernández. J., Valladares, M., Fuentes, R. 2006. Reorganization of cytoplasm in the zebrafish oocyte and egg during early steps of ooplasmic segregation. Dev. Dyn. 235: 656-671.

•Fernández  J., Toro J., Ubilla A. 2004. Confocal and video imaging of cytoskeleton dynamics in the leech zygot.e. Dev. Biol. 271: 59-74.

• Goicovich, E, Molina, C, Perez, P, Aguilera, S,, Fernández, J, Olea, N, Allende, C,. Leyton, R, Romo, R, Leyton L,  Gonzalez, M.J.. 2003.  Enhanced degradation of proteins of the basal lamina and   stroma by matrix metalloproteinases from the salivary glands of Sjögren syndrome patients. Arthritis and Rheumatism 48: 2573-2584.

•Fernández J, Cantillana V, Ubilla A. 2002. Reorganization and translocation of the ectoplasmic cytoskeleton in the leech zygote by condensation of cytasters and interactions of dynamic microtubules and actin filaments. Cell Motil. Cytoskeleton 53(3): 214-230.

•Cantillana V, Urrutia M, Ubilla A, Fernández, J. 2000.The complex dynamic network of microtubule and microfilament cytasters of the leech zygote. Dev. Biol. 228: 136-149

• Fuentes S, Retuert PJ, Ubilla A, Fernández J, González G. 2000. Relationship between composition and structure in chitosan-based hybrid films. Biomacromolecules. 1: 239-243.

• Fernández J, Olea N, Ubilla A, Cantillana V. 1998. Formation of polar cytoplasmic domains (teloplasms) in the leech egg is a three-step segregation process. Int. J. Dev. Biol. 42(2): 149-162.

•Fernández J, Roegiers F, Cantillana V, Sardet C. 1998. Formation and localization of cytoplasmic domains in leech and ascidian zygotes. Int. J. Dev. Biol. 42(8):1075-1084.

• Lepre M, Fernández J, Nicholls J. 1998. Reestablishment of direct synaptic connections between sensory axons and motoneurons after lesions of neonatal opposum CNS (Monodelphia domestica). Europ. J. Neurosc. 10: 2500-2510.

• Fernández J, Nicholls J. G. 1998. Fine structure and development of  dorsal root ganglion neurons and Schwann cells in the newborn opossum Monodelphis domestica. J. Comp. Neurol. 396: 338-350.

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