Grado:

Ph.D. en Microbiología e Ingeniería Genética, Universidad del Mediterráneo, Francia, 1996.

Tema de investigación o interés:

•Microbiología y Biotecnología-Biominería •Estudio de Quorum Sensing en la bacteria extremófila acidófila Acidithiobacillus  ferrooxidans con los objetivos de identificar las   funciones fisiológicas que controla y determinar su relevancia en la eficiencia de la biolixivación.

Proyectos:

•“Estudios del Quorum Sensing de tipo AI-1 mediado por el par génico afeR/afeI en la bacteria  extremófila acidófila Acidithiobacillus ferrooxidans”

FONDECYT

Investigador responsable: Prof. Nicolás Guiliani

Co-investigador: Prof. Carlos Jerez G.

Duración: 2004 - 2008

Publicaciones:

•Ramírez., P., Guiliani, N., Valenzuela, L., Beard, S., and Jerez, C.A.

Differential protein expression during growth of Acidithiobacillus ferrooxidans on ferrous iron, sulfur compounds or metal sulfides.

Appl. Environ. Microbiol. 70: 4491-4498.(2004)

•C. Farah, A. Banderas, C. Jerez, N. Guiliani

“Searching for physiological functions regulated by the quorum sensing autoinducer AI-1 promoted by afel/afeR genes in Acidithiobacillus ferroxidans”

In 15 th Internacional Biohydrometallurgy Symposium, Athens, Greece. Pp 151-159 (2003)

Appl. Environ. Microbiol 70: 3064 - 3072 (2004)

•Ramírez P., Guiliani N., Valenzuela, L., Jerez C. A

“Differential protein expression during growth of Acidithiobacillus ferrooxidans in ferrous iron, sulfur compounds and metal sulfides”

Appl. Environ. Microbiol 70: 4491 - 4498 (2004)

•Farah, C., Vera, M., Morin, D., Haras, D., Jerez, C.A. and Guiliani, N.

“Evidence of a functional quorum sensing type AI-1 system in the extremophilic bacterium Acidithiobacillus ferrooxidans”.

Appl. Environ. Microbiol. 71: 7033-7040 (2005)

•Guiliani N.

“Evidence for a functional quorum sensing type AI-1 system in the extremophilic bacterium Acidithiobacillus ferrooxidans”

Applied and Environmental Microbiology 71(11): 7033 - 7040 (2005)

•Valenzuela, L., Chi, A., Beard, S., Orell, A., Guiliani, N., Shabanowitz, J., Hunt, D.F. and Jerez, C.A.

“Genomics, metagenomics and proteomics in biomining microorganisms”.

Biotechnol. Adv. 24: 197-211 (2006)

•Vera, M., Pagliai, F., Guiliani, N., and Jerez, C.A.

“The chemolithoautotroph Acidithiobacillus ferrooxidans can survive under phosphate limiting conditions by the expression of a C-P lyase operon that allows it to grow in phosphonates”. Appl. Environ. Microbiol. 74:1829-1835 (2008).

•Frezza, M., Soulère, L., Reverchon, S., Guiliani, N., Jerez, C.A., Queneau, Y. and Doutheau, A.

“Synthetic homoserine lactone derived sulfonyl ureas as quorum sensing inhibitors in Vibrio fischeri bacteria”.

Bioorganic and Medicinal Chemistry. In press.

•Soulère, L., Guiliani, N., Jerez, C.A., Queneau Y. and Doutheau, A.

”Molecular insights into the quorum sensing in the bacterium Acidithiobacillus ferrooxidans through a molecular modeling study of the transcriptional regulator AfeR and the binding mode of long chain acyl homoserine lactones”

Computational Biology and Chemistry. Submitted.

•Ruiz, L., Valenzuela, S., Gonzalez, A., Frezza, M., Soulère, L., Rohwerder, T., Queneau, Y., Doutheau, A., Sand, W., Jerez, C.A., Guiliani, N.

“AHL communication is a widespread phenomenon in A. ferrooxidans species and seems to be involved in mineral-adhesion efficiency”

Hydrometallurgy. Submitted.

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